Telomerase Complex from Yeast Saccharomyces cerevisiae Contains a Biotinylated Component

نویسندگان

  • D.M. Shcherbakova
  • M.I. Zvereva
  • O.A. Dontsova
چکیده

Telomerase adds telomeric repeats to single-stranded DNA at the ends of the chromosomes. This enzyme is a ribonucleoprotein complex. Telomerase from yeast Saccharomyces cerevisiae consists of TLC1 RNA, which serves as a template for the synthesis of telomeric repeats, telomerase reverse transcriptase Est2p, and a number of accessory proteins (Est1p, Est3p, Ku70/Ku80, and Sm-complex). We found that the yeast telomerase complex contains a biotinylated component. The telomerase fraction containing biotinylated protein is active in vitro and constitutes a small part of the total amount of active telomerase isolated from cells. We speculate about the nature of the biotinylated component.

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عنوان ژورنال:

دوره 1  شماره 

صفحات  -

تاریخ انتشار 2009